Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CHAD antibody at 1:250 dilution.| Product Name | CHAD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHAD (chondroadherin)Chondroadherin is a cartilage matrix protein thought to mediate adhesion of isolated chondrocytes. The protein contains 11 leucine-rich repeats flanked by cysteine-rich regions. The chondroadherin messenger RNA is present in chondrocytes at all ages. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CHAD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (95%), Rat (95%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SLRR4A antibody CHAD antibody cartilage leucine-rich protein antibody chondroadherin antibody chondroadherin proteoglycan antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CHAD antibody at 1:250 dilution.| Positive Control | A549 , H1299 , HCT116 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
