Rabbit Polyclonal antibody to CHAF1BChromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]
Immunogen
A synthetic peptide corresponding to a C-terminal region of Human CHAF1B
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-PWB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Human: 100%
Concentration
1mg/ml
Purification
Protein A
Target Information
Gene Synonyms
MPP7 CAF1A CAF IP60 CAF1P60 CAF1 MPHOSPH7 CHAF1B
Target
CHAF1B
Molecular Weight(MW)
61 KDa (note)
Application
Application
IHC-P analysis of human kidney tissue using CHAF1B antibody at 4.0-8.0μg/ml.
Application
WB analysis of Jurkat cells using CHAF1B antibody at 5.0μg/ml. Lane A : marker Lane B : Jurkat cells
Positive Control
Jurkat
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.