Application
Sample (30 ug of whole cell lysate) A: HepG2 B: HepG2 nucleus 5% SDS PAGE diluted at 1:3000Application
CHD1L antibody immunoprecipitates CHD1L protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of CHD1L protein by 2 μg of CHD1L antibody 7.5% SDS-PAGE The immunoprecipitated CHD1L protein was detected by CHD1L antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Product Name | CHD1L antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHD1L (chromodomain helicase DNA binding protein 1-like)In response to DNA strand breaks, chromatin adopts a relaxed structure due to the addition of poly(ADP-ribose) (PAR) to chromatin proteins by PARP enzymes (see PARP1; MIM 173870), and this relaxation facilitates the repair of DNA damage. CHD1L interacts with PAR and has a role in chromatin relaxation following DNA damage (Ahel et al., 2009 [PubMed 19661379]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CHD1L. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (88%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ALC1 antibody CHDL antibody FLJ22530 antibody CHD1L antibody amplified in liver cancer 1 antibody chromodomain-helicase-DNA-binding protein 1-like antibody amplified in liver cancer protein 1 antibody chromodomain helicase DNA binding protein 1-like |
|---|---|
| Molecular Weight(MW) | 101 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HepG2 B: HepG2 nucleus 5% SDS PAGE diluted at 1:3000
Application
CHD1L antibody immunoprecipitates CHD1L protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of CHD1L protein by 2 μg of CHD1L antibody 7.5% SDS-PAGE The immunoprecipitated CHD1L protein was detected by CHD1L antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Positive Control | HepG2 , HepG2 nucleus |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
