Application
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using CHD-2 antibody at 1:100 dilution.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CHD2 antibody [N1N2], N-term diluted at 1:1000.| Product Name | CHD2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CHD-2 (chromodomain helicase DNA binding protein 2)The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CHD-2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, ChickenMouse (97%), Rat (98%), Chicken (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp547I1315 antibody DKFZp686E01200 antibody DKFZp781D1727 antibody FLJ38614 antibody CHD2 antibody ATP-dependent helicase CHD2 antibody chromodomain-helicase-DNA-binding protein 2 antibody CHD-2 antibody chromodomain helicase DNA binding protein |
|---|---|
| Target | CHD-2 |
| Molecular Weight(MW) | 211 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using CHD-2 antibody at 1:100 dilution.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CHD2 antibody [N1N2], N-term diluted at 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
