Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD3 antibody diluted at 1:1000.Application
CHD3 antibody detects CHD3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CHD3 protein stained by CHD3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
CHD3 antibody detects CHD3 protein at cytoplasm and nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CHD3 antibody diluted at 1:500.| Product Name | CHD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHD3 (chromodomain helicase DNA binding protein 3)This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CHD3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | MouseMouse (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | chromodomain helicase DNA binding protein 3 ZFH Mi2-ALPHA Mi-2a |
|---|---|
| Target | CHD3 |
| Molecular Weight(MW) | 227 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD3 antibody diluted at 1:1000.
Application
CHD3 antibody detects CHD3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CHD3 protein stained by CHD3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
CHD3 antibody detects CHD3 protein at cytoplasm and nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CHD3 antibody diluted at 1:500.| Positive Control | 293T , A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
