Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CHD4 antibody at 1:500 dilution.Application
Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody . Western blot analysis was performed using CHD4 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 5% SDS PAGE diluted at 1:1000| Product Name | CHD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHD4 (chromodomain helicase DNA binding protein 4)The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CHD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (91%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686E06161 antibody Mi-2b antibody Mi2-BETA antibody CHD4 antibody chromodomain-helicase-DNA-binding protein 4 antibody Mi-2 autoantigen 218 kDa protein antibody CHD-4 antibody ATP-dependent helicase CHD4 antibody chromodomain helicase DNA bind |
|---|---|
| Molecular Weight(MW) | 218 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CHD4 antibody at 1:500 dilution.
Application
Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody . Western blot analysis was performed using CHD4 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 5% SDS PAGE diluted at 1:1000| Positive Control | Jurkat , Raji , K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
