Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD5 antibody diluted at 1:1000.| Product Name | CHD5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHD5 (chromodomain helicase DNA binding protein 5)Changes in gene expression are associated with changes in chromatin structure, which can result from modification of histones by acetylation and methylation. In addition, SWI/SNF proteins can effect chromatin structure and gene expression by causing ATP-dependent disruption of the nucleosome and by enhancing binding of transcription factors. CHD5 belongs to a group of SWI/SNF proteins called CHD proteins, which contain a SWI/SNF-like helicase/ATPase domain, as well as a DNA-binding domain and a chromodomain that directly modifies chromatin structure (Thompson et al., 2003 [PubMed 12592387]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CHD5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (93%), Rat (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CHD-5 antibody chromodomain helicase DNA binding protein 5 antibody |
|---|---|
| Target | CHD5 |
| Molecular Weight(MW) | 223 kDa (note) |
| Cellular Localization | Nucleus |
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD5 antibody diluted at 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
