Application
Sample(30 μg of whole cell lysate) A:H1299 7.5% SDS PAGE diluted at 1:1000Application
CHFR antibody detects CHFR protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with CHFR antibody diluted by 1:500| Product Name | CHFR antibody , C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CHFR (checkpoint with forkhead and ring finger domains)E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CHFR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ10796 antibody FLJ33629 antibody RNF116 antibody RNF196 antibody CHFR antibody checkpoint with forkhead and RING finger domains protein antibody E3 ubiquitin-protein ligase CHFR antibody RING finger protein 196 antibody checkpoint with forkhead |
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| Target | CHFR |
| Molecular Weight(MW) | 73 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
Sample(30 μg of whole cell lysate) A:H1299 7.5% SDS PAGE diluted at 1:1000
Application
CHFR antibody detects CHFR protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with CHFR antibody diluted by 1:500| Positive Control | A431 , H1299 , NT2D1, *A549, *HCT116 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
