Application
CHI3L1 (Clone AT2C10) WB Result Lane 1: Recombinant CHI3L1 (50ng) Lane 2: SW480 Lysate (40ug) Lane 3: HepG2 Lysate (40ug) 1. Blocking: 5% Skim milk in TBST, 1hr 2. 1st Ab: 1:500, 2hr 3. 2nd Ab: 1:10000, 1hr 4. ECL| Product Name | CHI3L1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [AT2C10] to CHI3L1Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009] |
| Immunogen | Recombinant human CHI3L1 (22-383aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | AT2C10 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB: 1:250 - 1:1500: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GP39 ASRT7 HC-gp39 CGP-39 hCGP-39 YKL40 chitinase 3-like 1 (cartilage glycoprotein-39) YKL-40 YYL-40 GP-39 HCGP-3P |
|---|---|
| Target | CHI3L1 |
Application
CHI3L1 (Clone AT2C10) WB Result Lane 1: Recombinant CHI3L1 (50ng) Lane 2: SW480 Lysate (40ug) Lane 3: HepG2 Lysate (40ug) 1. Blocking: 5% Skim milk in TBST, 1hr 2. 1st Ab: 1:500, 2hr 3. 2nd Ab: 1:10000, 1hr 4. ECL| Application Notes | WB: 1:250 - 1:1500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol. |
