Application
WB analysis of lysates from (1) untreated and (2) irradiation-treated HeLa lysates (10 μg per lane) using Chk2 (phospho Ser33/35) antibody [EPR916(2)Y] at a dilution of 1:500.| Product Name | Chk2 (phospho Ser33/35) antibody [EPR916(2)Y] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR916(2)Y] to Chk2 (phospho Ser33/35)In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarc |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Serine 33/35 of human Chk2. |
| Modification | p-Ser33/35 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR916(2)Y |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | HuCds1 , hCds1 , CHK2 , checkpoint kinase 2 , PP1425 , LFS2 , RAD53 , CDS1 , CHEK2 |
|---|---|
| Target | CHK2 (phospho Ser33/35) |
| Tissue Specificity | This antibody detects Chk2 phosphorylated on Serine 33/35. |
Application
WB analysis of lysates from (1) untreated and (2) irradiation-treated HeLa lysates (10 μg per lane) using Chk2 (phospho Ser33/35) antibody [EPR916(2)Y] at a dilution of 1:500.| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Storage Buffer | pH: 7.4, Preservative: 0.01% Sodium azide. Constituents: 50% Glycerol, 0.05% BSA |
