Application
WB analysis of extracts (30ug) from HeLa cells untreated or treated with UV(15-30min) using Chk2 (phospho Ser516) antibody at a dilution of 1:500.| Product Name | Chk2 (phospho Ser516) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Chk2 (phospho S516)This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq] |
| Modification | p-Ser516 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Chk2 (phospho S516) |
|---|---|
| Cellular Localization | Present in lipid rafts in an unactive form. |
Application
WB analysis of extracts (30ug) from HeLa cells untreated or treated with UV(15-30min) using Chk2 (phospho Ser516) antibody at a dilution of 1:500.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS ( without Mg2+ and Ca2+ ), pH 7. 4 , 150mM NaCl , 0.02% sodium azide and 50% glycerol . |
