Application
Western blot analysis of CHK2 (pT432) expression in Jurkat (A); H9C2 (B) whole cell lysates.| Product Name | Chk2 (phospho Thr432) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Chk2 (phospho Thr432)In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarc |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CHK2 (pT432). The exact sequence is proprietary. |
| Modification | p-Thr432 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000 | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | checkpoint kinase 2 , PP1425 , HuCds1 , RAD53 , CDS1 , LFS2 , CHEK2 , CHK2 , hCds1 |
|---|---|
| Target | Chk2 (phospho Thr432) |
| Tissue Specificity | Recognizes endogenous levels of CHK2 (pT432) protein. |
Application
Western blot analysis of CHK2 (pT432) expression in Jurkat (A); H9C2 (B) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
