Application
Western blot analysis of Chk2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole cell lysates with| Product Name | Chk2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Chk2The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2 (1), p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2(6), were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53 (7,8). Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer (9). |
| Immunogen | Synthetic peptide: SRESDVEAQQSHGSSAC, corresponding to amino acids 2-18 of Human Chk2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blot at 0.5 to 1 μg/ml and a 60 kDa band can bedetected. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Unpurified |
| Gene Synonyms | HuCds1, CHK2, 114480, PP1425, LFS2, RAD53, CDS1, O96017, 11200, CHEK2 |
|---|---|
| Target | Chk2 |
| Cellular Localization | Nuclear |
Application
Western blot analysis of Chk2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole cell lysates with| Positive Control | Jurkat or K562 cell lysate. |
|---|---|
| Application Notes | Western blot at 0.5 to 1 μg/ml and a 60 kDa band can bedetected. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS with 0.02% sodium azide |
