Application
Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 ug/mL.Application
Western blot analysis of Chk2 expression in (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates with Chk2 antibody at 1 ug /ml.| Product Name | Chk2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Chk2In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarc |
| Immunogen | Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB: 1 ug/ml. ICC: 1 ug/ml | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HuCds1 , checkpoint kinase 2 , hCds1 , RAD53 , LFS2 , CHK2 , CDS1 , CHEK2 , PP1425 |
|---|---|
| Target | Chk2 |
Application
Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 ug/mL.
Application
Western blot analysis of Chk2 expression in (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates with Chk2 antibody at 1 ug /ml.| Application Notes | WB: 1 ug/ml. ICC: 1 ug/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
