Application
WB analysis of extracts from various cell lines using Chk2 antibody.Application
ICC/IF analysis of HeLa cells using Chk2 antibody (green) and DAPI (blue).Application
ICC/IF analysis of MCF7 cells using Chk2 antibody (green) and DAPI (blue).Application
IHC-P analysis of human stomach tissue using Chk2 antibody at a dilution of 1:100.| Product Name | Chk2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Chk2In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarc |
| Immunogen | Recombinant protein of human CHEK2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. ICC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:20 - 1:100 | |
| Species Reactivity | HumanMouseRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CHEK2 , RAD53 , HuCds1 , CDS1 , checkpoint kinase 2 , LFS2 , PP1425 , CHK2 , hCds1 |
|---|---|
| Target | Chk2 |
| Molecular Weight(MW) | 61 kDa (note) |
Application
WB analysis of extracts from various cell lines using Chk2 antibody.
Application
ICC/IF analysis of HeLa cells using Chk2 antibody (green) and DAPI (blue).
Application
ICC/IF analysis of MCF7 cells using Chk2 antibody (green) and DAPI (blue).
Application
IHC-P analysis of human stomach tissue using Chk2 antibody at a dilution of 1:100.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. ICC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:20 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
