Application
Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:2000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CHMP2B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | CHMP2B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHMP2B (chromatin modifying protein 2B)This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration. [provided by RefSeq] |
| Immunogen | Full length human CHMP2B Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineMouse (99%), Xenopus laevis (91%), Pig (99%), Chicken (94%), Rhesus Monkey (98%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CHMP2.5 antibody, DKFZp564O123 antibody, DMT1 antibody, VPS2-2 antibody, VPS2B antibody, CHMP2B antibody, chromatin modifying protein 2B antibody, vacuolar protein-sorting-associated protein 2-2 antibody, VPS2 homolog B antibody, charged multivesicular bod |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) |
| Cellular Localization | Cytoplasm , cytosol , Late endosome membrane |
Application
Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:2000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CHMP2B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
