Application
Sample (50 ug of whole cell lysate) A: mouse kidney 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using CHMP4C antibody at 1:500 dilution.Application
Sample (20 ug of whole cell lysate) A: HeLa B: HeLa cytoplasm 12% SDS PAGE diluted at 1:1000| Product Name | CHMP4C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHMP4C (chromatin modifying protein 4C)CHMP4C belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CHMP4C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chicken, Rhesus Monkey, BovineRat (91%), Pig (95%), Chicken (83%), Rhesus Monkey (99%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC22825 antibody, SNF7-3 antibody, Shax3 antibody, VPS32C antibody, CHMP4C antibody, vacuolar protein sorting-associated protein 32-3 antibody, chromatin modifying protein 4C antibody, chromatin-modifying protein 4c antibody, hVps32-3 antibody, hSnf7-3 an |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytosol , Late endosome membrane |
Application
Sample (50 ug of whole cell lysate) A: mouse kidney 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using CHMP4C antibody at 1:500 dilution.
Application
Sample (20 ug of whole cell lysate) A: HeLa B: HeLa cytoplasm 12% SDS PAGE diluted at 1:1000| Positive Control | HeLa , HeLa cytoplasm , mouse kidney |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
