Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CHMP5 antibody at 1:100 dilution.Application
CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis. Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (50 ug of whole cell lysate) A: mouse stomach 12% SDS PAGE diluted at 1:1000Application
Sample(30μg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 12% SDS PAGE diluted at 1:1000| Product Name | CHMP5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHMP5 (chromatin modifying protein 5)CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CHMP5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineRat (98%), Xenopus laevis (88%), Pig (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C9orf83 antibody, HSPC177 antibody, PNAS-2 antibody, SNF7DC2 antibody, Vps60 antibody, CHMP5 antibody, SNF7 domain containing 2 antibody, SNF7 domain-containing protein 2 antibody, chromatin-modifying protein 5 antibody, chromatin modifying protein 5 antib |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) |
| Cellular Localization | Cytoplasm , cytosol , Endosome membrane; Peripheral membrane protein , Cytoplasm , cytosol , Endosome membrane |
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using CHMP5 antibody at 1:100 dilution.
Application
CHMP5 antibody [N1C3] detects CHMP5 protein at cytoplasm by immunofluorescent analysis. Sample: HL-60 cells were fixed in ice-cold MeOH for 5 min. Green: CHMP5 protein stained by CHMP5 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (50 ug of whole cell lysate) A: mouse stomach 12% SDS PAGE diluted at 1:1000
Application
Sample(30μg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse stomach |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
