Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CHST15 antibody at 1:500 dilution.| Product Name | CHST15 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHST15 (carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15)Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CHST15. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (94%), Rat (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BRAG antibody, DKFZp781H1369 antibody, GALNAC4S-6ST antibody, KIAA0598 antibody, MGC34346 antibody, RP11-47G11.1 antibody, CHST15 antibody, B cell RAG associated protein (GALNAC4S-6ST) antibody, B-cell RAG-associated gene protein antibody, carbohydrate sul |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (5) |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein (Probable) |
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CHST15 antibody at 1:500 dilution.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
