Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed MCF-7, using CHST2 antibody at 1:500 dilution.| Product Name | CHST2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHST2 (carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2)N-acetylglucosamine-6-O-sulfotransferases, such as CHST2, catalyze the transfer of sulfate from 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) to position 6 of a nonreducing N-acetylglucosamine (GlcNAc) residue (Uchimura et al., 1998 [PubMed 9722682]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CHST2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rhesus Monkey, BovineMouse (98%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C6ST antibody, GST-2 antibody, GST2 antibody, Gn6ST-1 antibody, CHST2 antibody, carbohydrate sulfotransferase 2 antibody, carbohydrate (chondroitin 6/keratan) sulfotransferase 2 antibody, N-acetylglucosamine 6-O-sulfotransferase 1 antibody, galactose/N-ace |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) (2) |
| Cellular Localization | Golgi apparatus , trans-Golgi network membrane |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed MCF-7, using CHST2 antibody at 1:500 dilution.| Positive Control | NT2D1 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
