Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 10% SDS PAGE diluted at 1:3000Application
Immunofluorescence analysis of methanol-fixed MCF-7, using CHST7 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CHST7 antibody at 1:500 dilution.| Product Name | CHST7 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CHST7 (carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7)This gene belongs to the sulfotransferase gene family. Sulfotransferases generate sulfated glycosaminoglycan (GAG) moities during chondroitin sulfate biosynthesis. They create considerable structural diversity among chondroitin sulfates by transferring sulfate with remarkable specificity for the underlying oligosaccharide substrate. This gene product mainly transfers sulfate to N-acetylgalactosamine. The regulated expression of each member of this gene family may be an important determinant of sulfated GAGs expression and the associated function of chondroitin sulfates as regulators of many biologic processes. This gene is part of a gene cluster on chromosome Xp11.23. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CHST7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (92%), Rat (91%), Rhesus Monkey (100%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C6ST-2 antibody, CHST7 antibody, chondroitin 6-sulfotransferase-2 antibody, carbohydrate sulfotransferase 7 antibody, glcNAc6ST-4 antibody, gn6st-4 antibody, GST-5 antibody, N-acetylglucosamine 6-O-sulfotransferase 4 antibody, galactose/N-acetylglucosamine |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (2) |
| Cellular Localization | Golgi apparatus membrane |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 10% SDS PAGE diluted at 1:3000
Application
Immunofluorescence analysis of methanol-fixed MCF-7, using CHST7 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CHST7 antibody at 1:500 dilution.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
