Application
Sample (50 µg of whole cell lysate) A: Rat muscle 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody diluted at 1:1000 and competitor's antibody (SC-7943) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
cIAP1 antibody detects cIAP1 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. cIAP1 antibody diluted at 1:500.| Product Name | cIAP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to cIAP1 (baculoviral IAP repeat-containing 2)The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human cIAP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Rhesus MonkeyDog (89%), Rhesus Monkey (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | API1 antibody, HIAP2 antibody, Hiap-2 antibody, MIHB antibody, RNF48 antibody, c-IAP1 antibody, cIAP1 antibody, BIRC2 antibody, apoptosis inhibitor 1 antibody, IAP-2 antibody, TNFR2-TRAF-signaling complex protein 2 antibody, RING finger protein 48 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Sample (50 µg of whole cell lysate) A: Rat muscle 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody diluted at 1:1000 and competitor's antibody (SC-7943) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
cIAP1 antibody detects cIAP1 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. cIAP1 antibody diluted at 1:500.| Positive Control | NCI-H929 , Jurkat , rat muscle , *Mouse B cell , *Mouse T cell , *Mouse Thymocyte |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
