Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using CIDEC antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using CIDEC antibody at 1:500 dilution.| Product Name | CIDEC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CIDEC (cell death-inducing DFFA-like effector c)DNA fragmentation factor (DFF) induces the fragmentation of DNA associated with apoptosis. A novel family of cell death-inducing DFF45 (MIM 601882)-like effectors (CIDEs), including CIDEC, can also promote apoptosis (Liang et al., 2003 [PubMed 12429024]).[supplied by OMIM] |
| Immunogen | Full length human CIDEC Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rat, Pig, SheepRat (80%), Pig (83%), Sheep (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CIDE-3 antibody, FLJ20871 antibody, FSP27 antibody, CIDEC antibody, fat specific protein 27 antibody, cell death activator CIDE-3 antibody, cell death-inducing DFFA-like effector c antibody |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using CIDEC antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using CIDEC antibody at 1:500 dilution.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
