Application
ChIP assays were performed using NALM cells, CIITA antibosy and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 μg of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Figure 1 shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).| Product Name | CIITA antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CIITA |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ChIP |
| ChIP (5-7 µg/IP) | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | class II, major histocompatibility complex, transactivator, MHC2TA, CIITAIV, NLRA, C2TA |
|---|---|
| Target | CIITA |
Application
ChIP assays were performed using NALM cells, CIITA antibosy and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 μg of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Figure 1 shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).| Application Notes | ChIP (5-7 µg/IP) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
