Application
Immunohistochemical analysis of paraffin-embedded MDAMB-468 xenograft, using CIP29 antibody at 1:100 dilution.Application
CIP29 antibody [N1C3] detects CIP29 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CIP29 protein stained by CIP29 antibody [N1C3] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Sample(30 ug whole cell lysate) A:293T B:A431 C:H1299 12% SDS PAGE diluted at 1:1000| Product Name | CIP29 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CIP29This gene encodes a protein that is upregulated in response to various cytokines. The encoded protein may play a role in cell cycle progression. A translocation between this gene and the myeloid/lymphoid leukemia gene, resulting in expression of a chimeric protein, has been associated with acute myelomonocytic leukemia. Pseudogenes exist on chromosomes 7 and 8. Alternatively spliced transcript variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CIP29. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, BovineMouse (95%), Rat (95%), Chicken (80%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CIP29 antibody, HCC1 antibody, HSPC316 antibody, MGC14726 antibody, THO1 antibody, SARNP antibody, nuclear protein Hcc-1 antibody, SAP domain-containing ribonucleoprotein antibody, cytokine-induced protein of 29 kDa antibody, hepatocellular carcinoma 1 ant |
|---|---|
| Target | CIP29 |
| Molecular Weight(MW) | 24 kDa (note) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded MDAMB-468 xenograft, using CIP29 antibody at 1:100 dilution.
Application
CIP29 antibody [N1C3] detects CIP29 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CIP29 protein stained by CIP29 antibody [N1C3] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Sample(30 ug whole cell lysate) A:293T B:A431 C:H1299 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
