Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CIRBP antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CIRBP antibody [N1C3] detects CIRBP protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CIRBP protein stained by CIRBP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | CIRBP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CIRBP (cold inducible RNA binding protein) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CIRBP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (94%), Rat (94%), Chicken (90%), Rhesus Monkey (100%), Chimpanzee (97%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CIRP antibody, CIRBP antibody, cold-inducible RNA-binding protein antibody, A18 hnRNP antibody, cold inducible RNA-binding protein antibody, glycine-rich RNA-binding protein CIRP antibody, glycine-rich RNA binding protein antibody, cold inducible RNA bindi |
|---|---|
| Molecular Weight(MW) | 19 kDa (note) (2) |
| Cellular Localization | Nucleus , nucleoplasm |
Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CIRBP antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CIRBP antibody [N1C3] detects CIRBP protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CIRBP protein stained by CIRBP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
