Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CIS antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using CIS antibody at 1:100 dilution.Application
CIS antibody [C2C3], C-term detects CISH protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE CIS antibody [C2C3], C-term dilution: 1:1000Application
Sample (1 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:10000| Product Name | CIS antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CIS (cytokine inducible SH2-containing protein)The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CIS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Pig, Rhesus Monkey, BovineMouse (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CIS antibody, CIS-1 antibody, G18 antibody, SOCS antibody, CISH antibody, suppressor of cytokine signaling antibody, cytokine-inducible inhibitor of signaling type 1B antibody, cytokine-inducible SH2-containing protein antibody, cytokine inducible SH2-cont |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (2) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CIS antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using CIS antibody at 1:100 dilution.
Application
CIS antibody [C2C3], C-term detects CISH protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE CIS antibody [C2C3], C-term dilution: 1:1000
Application
Sample (1 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:10000| Positive Control | HeLa , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
