Application
CIZ1 antibody [N2C1], Internal detects CIZ1 protein by Western blot analysis. A. 30 μg HeLa whole cell lysate/extract 7.5 % SDS-PAGE CIZ1 antibody [N2C1], Internal dilution: 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CIZ1 antibody at 1:200 dilution.Application
CIZ1 antibody immunoprecipitates CIZ1 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of CIZ1 protein by 2.5 μg of CIZ1 antibody 7.5% SDS-PAGE The immunoprecipitated CIZ1 protein was detected by CIZ1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CIZ1 antibody at 1:500 dilution.| Product Name | CIZ1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CIZ1 (CDKN1A interacting zinc finger protein 1)May regulate the subcellular localization of CIP/WAF1. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CIZ1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LSFR1 antibody NP94 antibody ZNF356 antibody CIZ1 antibody CDKN1A-interacting zinc finger protein 1 antibody nuclear protein NP94 antibody zinc finger protein 356 antibody cip1-interacting zinc finger protein antibody CDKN1A interacting zinc finger |
|---|---|
| Molecular Weight(MW) | 100 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
CIZ1 antibody [N2C1], Internal detects CIZ1 protein by Western blot analysis. A. 30 μg HeLa whole cell lysate/extract 7.5 % SDS-PAGE CIZ1 antibody [N2C1], Internal dilution: 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CIZ1 antibody at 1:200 dilution.
Application
CIZ1 antibody immunoprecipitates CIZ1 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of CIZ1 protein by 2.5 μg of CIZ1 antibody 7.5% SDS-PAGE The immunoprecipitated CIZ1 protein was detected by CIZ1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CIZ1 antibody at 1:500 dilution.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
