| Product Name | c-Jun antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to c - JunThe human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. c-Jun (previously known as the Fos-binding protein p39) and c-Fos form a complex in the nucleus. AP 1 (activating protein-1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto-oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. |
| Immunogen | Synthetic peptide: PSESGPYGYSNPKILKQS, corresponding to amino acids 20-37 of Human c-Jun |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1:100 to 1:250 is recommended. Detects a band of approximately 41 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseMonkeyPigRatSheepBovine |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cJun, JUN, AP1, 165160, P05412, 3725, c Jun |
|---|---|
| Target | c-Jun |
| Cellular Localization | Nuclear |
| Positive Control | GTX14643: 3T6 Swiss albino nuclear extract |
|---|---|
| Application Notes | WB: Use at a dilution of 1:100 to 1:250 is recommended. Detects a band of approximately 41 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.2% gelatin, 0.01% thiomerosal, and 0.1% sodium azide |
