Application
Western blot analysis of extracts from HeLa cells, using CKI-alpha Antibody. The lane on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CKI-alpha Antibody. The picture on the right is treated with the synthesized peptide.Application
Immunofluorescence analysis of HeLa cells, using CKI-alpha Antibody. The picture on the right is treated with the synthesized peptide.IHC
a CK1α was strongly activated in the outer and inner enamel epithelium and stratum intermedium, with ameloblasts and odontoblasts showing high expression in the F group. b In contrast, CK1α nearly was not detected in the control group. Scale bar = 100 μm.IHC
a, b CK1α activation was still clearly evident in the inner and outer enamel epithelium, ameloblasts, odontoblasts and mesenchyme of the F group. c, d CK1α expression patterns were mildly in the control group.| Product Name | CKI- alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CKI- alphaCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CKI-alpha. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | kinase CK1-alpha, EC2.7.11.1, KC1A, kinase CK1alpha, Casein kinase I, CKI-alpha, CKIalpha, CK1alpha, CKI alpha, CK1-alpha, alpha isoform, CSNK1A1, EC 2.7.11.1, CK1 alpha, kinase CK1 alpha |
|---|---|
| Target | CKI- alpha |
| Molecular Weight(MW) | 38 kDa (note) |
| Tissue Specificity | CKI-alpha Antibody detects endogenous levels of total CKI-alpha protein. |
| Cellular Localization | Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. |
Application
Western blot analysis of extracts from HeLa cells, using CKI-alpha Antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CKI-alpha Antibody. The picture on the right is treated with the synthesized peptide.
Application
Immunofluorescence analysis of HeLa cells, using CKI-alpha Antibody. The picture on the right is treated with the synthesized peptide.
IHC
a CK1α was strongly activated in the outer and inner enamel epithelium and stratum intermedium, with ameloblasts and odontoblasts showing high expression in the F group. b In contrast, CK1α nearly was not detected in the control group. Scale bar = 100 μm.
IHC
a, b CK1α activation was still clearly evident in the inner and outer enamel epithelium, ameloblasts, odontoblasts and mesenchyme of the F group. c, d CK1α expression patterns were mildly in the control group.| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC:1:50~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
