| Product Name | CKII alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CKII alpha prime polypeptideCKII is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The gene for this protein is found on chromosome 20, but a related transcribed pseudogene is found on chromosome 11. Three transcript variants have been found, encoding two different isoforms. Protein kinase CKII is recognized as one of the key cellular signals for cell growth and proliferation; it also has an important role in apoptosis. |
| Immunogen | Immunogen was a synthetic peptide, which represented a portion of human Casein kinase 2, alpha 2 polypeptide encoded within exon 11 (LocusLink ID 1459). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| For IP: Use at dilution of 4-10 μg/mg of lysate. For WB: Use at a dilution of 0.2 - 1.0 μg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa). Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CK2A2, CSNK2A1, CKII alpha, 115442, CSNK2A2, 1459, P19784 |
|---|---|
| Target | CKII alpha |
| Application Notes | For IP: Use at dilution of 4-10 μg/mg of lysate. For WB: Use at a dilution of 0.2 - 1.0 μg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa). Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 0.1ml Tris-citrate/phosphate, pH 7-8, containing 0.1% sodium azide |
