Application
Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C| Product Name | c-Kit (phospho Tyr568/Tyr570) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to c - Kit ( phospho Y568 + Y570 )c Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c Kit proto oncogene. c Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosines 568 and 570 of c Kit allows binding and activation of Src family kinases. |
| Immunogen | Synthetic phosphopeptide derived from the region of human c-Kit that contains tyrosines 568 and 570. |
| Modification | p-Tyr568/Tyr570 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1/1000 Detects a band of approximately 145 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CKit, KIT, CD117, P10721, SCFR, C-Kit, PBT, 164920, 3815, c Kit, c-Kit (phospho Y568/Y570), c-Kit (phospho Tyr568/Tyr570), c-Kit (pY568/pY570), c-Kit (pTyr568/pTyr570), c-Kit phospho Y568/Y570, c-Kit phospho Tyr568/Tyr570, Phospho c-Kit (Y568/Y570), Phosp |
|---|---|
| Target | c-Kit |
| Cellular Localization | Type I membrane protein. |
Application
Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C| Positive Control | M07e cells + or - SCF |
|---|---|
| Application Notes | WB: 1/1000 Detects a band of approximately 145 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA (IgG, protease free). pH 7.3 |
