Application
ApplicationApplication
Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C| Product Name | c-Kit (phospho Tyr730) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to c - Kit ( phospho Y730 )c Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c Kit proto-oncogene. c Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 730 of c Kit allows binding of PLC gamma and signaling to multiple pathways. |
| Immunogen | Synthetic phosphopeptide derived from a region of human c Kit that contains tyrosine 730. |
| Modification | p-Tyr730 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1/1000. Detects a band of approximately 145 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseDogRatBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CKit, KIT, CD117, P10721, SCFR, C-Kit, PBT, 164920, 3815, c Kit, c-Kit (phospho Y730), c-Kit (phospho Tyr730), c-Kit (pY730), c-Kit (pTyr730), c-Kit phospho Y730, c-Kit phospho Tyr730, Phospho c-Kit (Y730), Phospho c-Kit (Tyr730), Phospho c-Kit (pY730), Ph |
|---|---|
| Target | c-Kit |
| Cellular Localization | Type I membrane protein. |
Application
Application
Application
Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C| Positive Control | M07e cells + or - SCF |
|---|---|
| Application Notes | WB: 1/1000. Detects a band of approximately 145 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA |
