Application
Extracts of M07e cells unstimulated (1) or stimulated with 250ng/mL SCF (2-5) incubated with c-Kit [pY823] antibody, after: no peptide (1, 2), non-phosphorylated peptide (3), generic phosphotyrosine-containing peptide (4), phosphopeptide immunogen (5)| Product Name | c-Kit (phospho Tyr823) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to c - Kit ( phospho Y823 )c Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c Kit proto-oncogene. c Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Tyrosine 823 of c-Kit has been identified as a major autophosphorylation site, but its function is still under study. |
| Immunogen | Synthetic phosphopeptide derived from a region of human c Kit that contains tyrosine 823. |
| Modification | p-Tyr823 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 - 1.0 μg/ml. Detects a band of approximately 145 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseDogRatBovine |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CKit, KIT, CD117, P10721, SCFR, C-Kit, PBT, 164920, 3815, c Kit, c-Kit (phospho Y823), c-Kit (phospho Tyr823), c-Kit (pY823), c-Kit (pTyr823), c-Kit phospho Y823, c-Kit phospho Tyr823, Phospho c-Kit (Y823), Phospho c-Kit (Tyr823), Phospho c-Kit (pY823), P |
|---|---|
| Target | c-Kit |
| Cellular Localization | Type I membrane protein. |
Application
Extracts of M07e cells unstimulated (1) or stimulated with 250ng/mL SCF (2-5) incubated with c-Kit [pY823] antibody, after: no peptide (1, 2), non-phosphorylated peptide (3), generic phosphotyrosine-containing peptide (4), phosphopeptide immunogen (5)| Positive Control | M07e cells treated with SCF |
|---|---|
| Application Notes | WB: Use at a concentration of 0.1 - 1.0 μg/ml. Detects a band of approximately 145 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA. pH 7.4 |
