Application
Formalin-fixed and paraffin embedded rat intestine labeled with Rabbit Anti-c-Kit Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB stainingApplication
Image provided by the Free Sample Program. Mouse pancreatic cancer tissue lysates probed with Rabbit Anti-c-Kit Polyclonal Antibody, Unconjugated at 1:1000 overnight at 4˚C. Followed by conjugation to secondary antibody and DAB development.Application
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-c-Kit Polyclonal Antibody at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.Application
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-c-Kit Polyclonal Antibody at 1:200 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.| Product Name | c-Kit antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to c-KitThis gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide derived from human c-Kit |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:1000, IHC-P: 1:50 - 1:500, ICC/IF: 1:50 - 1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | CD117 , KIT proto-oncogene receptor tyrosine kinase , KIT , SCFR , C Kit , PBT |
|---|---|
| Target | c-Kit |
Application
Formalin-fixed and paraffin embedded rat intestine labeled with Rabbit Anti-c-Kit Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Application
Image provided by the Free Sample Program. Mouse pancreatic cancer tissue lysates probed with Rabbit Anti-c-Kit Polyclonal Antibody, Unconjugated at 1:1000 overnight at 4˚C. Followed by conjugation to secondary antibody and DAB development.
Application
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-c-Kit Polyclonal Antibody at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Application
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-c-Kit Polyclonal Antibody at 1:200 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.| Application Notes | WB: 1:500 - 1:1000, IHC-P: 1:50 - 1:500, ICC/IF: 1:50 - 1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
