Application
Western blot analysis of CLASP2 Antibody (Y1019) in mouse spleen tissue lysates (35ug/lane). CLASP2 (arrow) was detected using the purified Pab.Application
CLASP2 Antibody (Y1019) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CLASP (arrow) using rabbit polyclonal CLASP Antibody (Y1019) . 293 cell lysates transfected with the ACADL gene (Lane 1) and with the GFP-CLASP (Lane 2).| Product Name | CLASP2 antibody, Y1019 |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CLASP2 (Y1019).Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. |
| Immunogen | KLH conjugated synthetic peptide between 1005~1034 amino acids from the Y1019 region of human CLASP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cytoplasmic linker-associated protein 2, Protein Orbit homolog 2, hOrbit2, Cytoplasmic linkerassociated protein 2, CLIP-associating protein 2, CLIPassociating protein2, KIAA0627, CLASP2, Cytoplasmic linkerassociated protein2, CLASP2, Protein Orbit homolog2 |
|---|---|
| Target | CLASP2 |
| Molecular Weight(MW) | 141.133 kDa (note) |
| Cellular Localization | Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus e |
Application
Western blot analysis of CLASP2 Antibody (Y1019) in mouse spleen tissue lysates (35ug/lane). CLASP2 (arrow) was detected using the purified Pab.
Application
CLASP2 Antibody (Y1019) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CLASP (arrow) using rabbit polyclonal CLASP Antibody (Y1019) . 293 cell lysates transfected with the ACADL gene (Lane 1) and with the GFP-CLASP (Lane 2).| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
