Application
Western blot analysis of extracts from COLO cells treated with EGF using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Claudin 3 (phospho Tyr219) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Claudin 3 (phospho Tyr219)Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 219 (R-K-D-Y(p)-V) derived from Human Claudin 3. |
| Modification | p-Tyr219 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C7orf1 CPETR2 RVP1 CPE-R2 claudin 3 CLDN3 HRVP1 |
|---|---|
| Target | Claudin 3 (phospho Tyr219) |
| Tissue Specificity | The antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine 219. |
Application
Western blot analysis of extracts from COLO cells treated with EGF using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
