Rabbit polyclonal to Claudin 5 predilutedClaudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water, etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.
Immunogen
Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Mouse) (C terminal).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-P
IHC-P: Ready to use. Incubate for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 miniutes followed by cooling at room temperature for 20 minutes. Optimal dilutions/concentrations should be determined by the end user.:
staining Claudin 5 in human lung by Immunohistochemistry (FFPE-sections)
Positive Control
Lung
Application Notes
IHC-P: Ready to use. Incubate for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 miniutes followed by cooling at room temperature for 20 minutes. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution and store at 4°C. Do not freeze.
Storage Buffer
PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide