Application
WB analysis of 293T cells using CLCN3 antibody at 1.0μg/ml.Application
IHC-P analysis of human brain tissue using CLCN3 antibody at 4.0-8.0μg/ml.Application
WB analysis of HepG2 cells using CLCN3 antibody at 1.25μg/ml.| Product Name | CLCN3 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CLCN3This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
| Immunogen | A synthetic peptide corresponding to a C-terminal region of Human CLCN3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 91%; Zebrafish: 100% |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | CLCN3 CLC3 |
|---|---|
| Target | CLCN3 |
| Molecular Weight(MW) | 91 KDa (note) |
Application
WB analysis of 293T cells using CLCN3 antibody at 1.0μg/ml.
Application
IHC-P analysis of human brain tissue using CLCN3 antibody at 4.0-8.0μg/ml.
Application
WB analysis of HepG2 cells using CLCN3 antibody at 1.25μg/ml.| Positive Control | 293T , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
