CLCN3 plays an important role in the cell proliferation of vascular smooth muscle cells. The PDZ-binding isoform of CLCN3 resides in the Golgi where it co-localizes with a small amount of CFTR (cystic fibrosis transmembrane conductance regulator).1) Salazar, G., et al., (2004) J. Biol. Chem. 279 (24), 25430-25439.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLCN3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
CLCN3 antibody can be used for detection of CLCN3 by ELISA at 1:1562500. CLCN3 antibody can be used for detection of CLCN3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in IHC on Human Brain.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
CLCN3 antibody can be used for detection of CLCN3 by ELISA at 1:1562500. CLCN3 antibody can be used for detection of CLCN3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CLCN3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt