Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CLIC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.| Product Name | CLIC4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CLIC4 (chloride intracellular channel 4)Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CLIC4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Rhesus Monkey, BovineMouse (98%), Rat (96%), Zebrafish (85%), Xenopus laevis (87%), Rhesus Monkey (99%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CLIC4L antibody DKFZp566G223 antibody FLJ38640 antibody H1 antibody MTCLIC antibody huH1 antibody p64H1 antibody CLIC4 antibody chloride intracellular channel 4 like antibody intracellular chloride ion channel protein p64H1 antibody chloride intr |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) |
| Cellular Localization | Cytoplasm , Cytoplasmic vesicle membrane; Single-pass membrane protein , Nucleus , Cell membrane; Single-pass membrane protein , Cytoplasm , Cytoplasmic vesicle membrane , Nucleus , Cell membrane |
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CLIC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.| Positive Control | HeLa , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
