Application
Western blot analysis of anti-CLIC5 Antibody in K562 cell line lysates (35ug/lane). CLIC5(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with CLIC5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CLIC5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CLIC5Chloride intracellular channels are involved in chloride ion transport within various subcellular compartments. CLIC5 specifically associates with the cytoskeleton of placenta microvilli (Berryman and Bretscher, 2 [PubMed 1793131]).[supplied by OMIM] |
| Immunogen | human recombinant CLIC5 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Chloride intracellular channel protein 5 |
|---|---|
| Target | CLIC5 |
| Molecular Weight(MW) | 46.469 kDa (note) |
| Cellular Localization | Associates with the cortical actin cytoskeleton. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Colocalized with AKAP9 at the Golgi apparatus as well as, to a lesser extent, the centrosome. |
Application
Western blot analysis of anti-CLIC5 Antibody in K562 cell line lysates (35ug/lane). CLIC5(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with CLIC5 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
