Application
CLK3 antibody detects CLK3 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CLK3 antibody diluted by 1:1000.Application
CLK3 antibody detects CLK3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CLK3 protein stained by CLK3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | CLK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CLK3 (CDC-like kinase 3)This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CLK3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Chimpanzee, BovineMouse (100%), Rat (100%), Chicken (88%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PHCLK3 CDC-like kinase 3 PHCLK3/152 |
|---|---|
| Target | CLK3 |
| Molecular Weight(MW) | 59 kDa (note) (2) |
| Cellular Localization | Nucleus , Cytoplasm , Acrosome |
Application
CLK3 antibody detects CLK3 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CLK3 antibody diluted by 1:1000.
Application
CLK3 antibody detects CLK3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CLK3 protein stained by CLK3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
