Application
Western blot analysis of Clock expression in Jurkat cell lysate (Lane 1 & 2).| Product Name | CLOCK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CLOCKClock is a basic helix-loop helix (bHLH) transcription factor that regulates circadian rhythms and sleep cycles. Clock contain a DNA-binding domain, a protein dimerization domain that heterodimerizes with BMAL1 and a glutamine-rich C terminus region. Clock dimerization with BMAL helps regulate nuclear translocation and binding of Clock to the E box elements stimulates circadian genes transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes the 100 kDa and the ~62 kDa isoforms of CLOCK in samples from human, mouse and rat origins. Reactivity to other species has not been tested.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Circadian Locomoter Output Cycles Kaput clock homolog |
|---|---|
| Target | CLOCK |
| Tissue Specificity | This antibody is specific for CLOCK. |
| Cellular Localization | Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent. Phosphorylated form located in the nucleus predominantly between C12 and C21. Nonphosphorylated form found only in the cytoplasm. |
Application
Western blot analysis of Clock expression in Jurkat cell lysate (Lane 1 & 2).| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes the 100 kDa and the ~62 kDa isoforms of CLOCK in samples from human, mouse and rat origins. Reactivity to other species has not been tested.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
