Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CLOCK antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
CLOCK antibody [C2C3], C-term detects CLOCK protein at cytoplasm and nucleus in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. CLOCK antibody [C2C3], C-term diluted at 1:500.Application
CLOCK antibody [C2C3], C-term detects CLOCK protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CLOCK antibody [C2C3], C-term diluted at 1:500.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:500| Product Name | CLOCK antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CLOCK (clock homolog (mouse))This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human CLOCK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, Sheep, BovineChicken (80%), Sheep (89%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | KAT13D antibody KIAA0334 antibody bHLHe8 antibody CLOCK antibody circadian locomoter output cycles kaput protein antibody class E basic helix-loop-helix protein 8 antibody circadian locomoter output cycles protein kaput antibody clock homolog (mouse |
|---|---|
| Molecular Weight(MW) | 95 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CLOCK antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
CLOCK antibody [C2C3], C-term detects CLOCK protein at cytoplasm and nucleus in rat lung by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. CLOCK antibody [C2C3], C-term diluted at 1:500.
Application
CLOCK antibody [C2C3], C-term detects CLOCK protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CLOCK antibody [C2C3], C-term diluted at 1:500.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:500| Positive Control | Mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
