Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CLP1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded HS578T xenograft, using CLP1 antibody at 1:100 dilution.| Product Name | CLP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CLP1 (CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae))Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CLP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (99%), Rat (99%), Xenopus laevis (82%), Chicken (98%), Rhesus Monkey (92%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HEAB antibody hClp1 antibody CLP1 antibody pre-mRNA cleavage complex II protein Clp1 antibody homolog of yeast CFIA subunit Clp1p antibody polynucleotide kinase Clp1 antibody polyribonucleotide 5'-hydroxyl-kinase Clp1 antibody ATP/GTP-binding protei |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CLP1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded HS578T xenograft, using CLP1 antibody at 1:100 dilution.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
