Application
Sample(30 μg of whole cell lysate) A:293T B:HeLa S3 7.5% SDS PAGE diluted at 1:1500Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CMAS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | CMAS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CMAS (cytidine monophosphate N-acetylneuraminic acid synthetase)The enzyme encoded by this gene catalyzes the activation of Neu5Ac to Cytidine 5-prime-monophosphate N-acetylneuraminic acid (CMP-Neu5Ac), which provides the substrate required for the addition of sialic acid. Sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. The pattern of cell surface sialylation is highly regulated during embryonic development, and changes with stages of differentiation. Studies of a similar murine protein suggest that this protein localizes to the nucleus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CMAS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, Chimpanzee, BovineMouse (98%), Rat (97%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CMAS antibody cytidine 5'-monophosphate N-acetylneuraminic acid synthetase antibody CMP-NeuNAc synthase antibody N-acylneuraminate cytidylyltransferase antibody CMP-N-acetylneuraminic acid synthase antibody CMP-Neu5Ac synthetase antibody CMP-N-acetyl |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample(30 μg of whole cell lysate) A:293T B:HeLa S3 7.5% SDS PAGE diluted at 1:1500
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CMAS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | 293T , HeLaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
