Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] diluted at 1:500.Application
c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody diluted by 1:1000.Application
Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of MET antibody [GT556] . Western blot analysis was performed using c-Met antibody [GT556] . EasyBlot anti-Mouse IgG was used as a secondary reagent.Application
c-Met antibody [GT556] detects c-Met protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. c-Met antibody [GT556] diluted at 1:100.Application
c-Met antibody [GT556] detects c-Met protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. c-Met antibody [GT556] diluted at 1:100.| Product Name | c-Met antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT556] to c-Met (met proto-oncogene (hepatocyte growth factor receptor))The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human c-Met. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT556 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| WB:1:500~1:2000 IP:1:100~1:500 IHC-p:1:50~1:200 |
|
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | c-Met RCCP2 AUTS9 met proto-oncogene (hepatocyte growth factor receptor) HGFR |
|---|---|
| Target | c-Met |
| Molecular Weight(MW) | 156 kDa (note) (3) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] diluted at 1:500.
Application
c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody diluted by 1:1000.
Application
Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of MET antibody [GT556] . Western blot analysis was performed using c-Met antibody [GT556] . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Application
c-Met antibody [GT556] detects c-Met protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. c-Met antibody [GT556] diluted at 1:100.
Application
c-Met antibody [GT556] detects c-Met protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. c-Met antibody [GT556] diluted at 1:100.| Positive Control | A431 , HeLa , HepG2 |
|---|---|
| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IHC-p:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
