Application
Sample(30 ug whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:500Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000Application
CMPK1 antibody [N1C3] detects CMPK1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CMPK1 protein stained by CMPK1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | CMPK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CMPK1 (cytidine monophosphate (UMP-CMP) kinase 1, cytosolic)Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis (Liou et al., 2002 [PubMed 11912132]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CMPK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (98%), Xenopus laevis (85%), Chicken (91%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CMK antibody CMPK antibody UMK antibody UMP-CMPK antibody UMPK antibody CMPK1 antibody UMP-CMP kinase antibody uridine monophosphate kinase antibody uridine monophosphate/cytidine monophosphate kinase antibody UMP/CMP kinase antibody deoxycytidyl |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample(30 ug whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:500
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000
Application
CMPK1 antibody [N1C3] detects CMPK1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CMPK1 protein stained by CMPK1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
