Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYC.Application
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MYC mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-MYC mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MYC mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-MYC mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-MYC mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-MYC mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MYC mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MYC mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MYC mouse monoclonal antibody.Application
Anti-MYC mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with MYCApplication
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MYC monoclonal antibody.| Product Name | c-Myc antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Mouse monoclonal antibody [5G3] to c-MycParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. |
| Immunogen | Recombinant fragment expressed in E.coli corresponding to amino acids 1-454 of human c-Myc |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 5G3 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| ICC/IF (1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | c Myc cMyc MYC c-Myc |
|---|---|
| Target | c-Myc |
| Tissue Specificity | is specific for amino acids 1-454 of human c-Myc |
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYC.
Application
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MYC mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-MYC mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MYC mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-MYC mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-MYC mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-MYC mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MYC mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MYC mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MYC mouse monoclonal antibody.
Application
Anti-MYC mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with MYC
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MYC monoclonal antibody.| Application Notes | ICC/IF (1:100): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
